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In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of gammaglutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 4)

Further, PEGylated LNPs that efficiently encapsulate siRNA can be used against SNCA as they are shown to efficiently penetrate the BBB (Figure 4) and sustain gene knockdown in dopaminergic neurons

FIGURE 2 In contrast, with the administration of L-NAME, there was a moderate generalized irregularity in the diameter of blood vessels with moderate atrophy of the optic disc, faint presentation of the choroidal blood vessels appears immediately, and these lesions (Figure 2, IN, IIN) rapidly progress (Figure 3, AC